Mapping of RhoGAP double-knockdown TCs to RhoGTPase overexpression TCs. (A–C) Significance score for mapping of all single- and double-knockdown RhoGAP TCs to the RhoF30L, RacF28L, and Cdc42Y32A TCs, respectively. The color scale ranges from blue (highly significant mapping) to red (low significance). For example, the RacGAP50C/RacGAP84C double-knockdown TC was mapped to RacF28L with high significance (see also Supplemental Table 3). (D) Robustness of classification to exclusion of data using jackknifing. For each single- and double-knockdown TC, 100 random samples consisting of X% (X = 30, 50, 70, 90) of the cells from that TC were selected and classified to the set of overexpression TCs. A consistency score was assigned based on the fraction of random samples correctly classified. Single- and double-knockdowns were binned into groups depending on the P-score of the true classification. Mean and standard deviations of consistency scores are shown here for the three groups defined by largest P-scores (see graph legend). Most importantly, high-confidence classifications are extremely robust to data exclusion (top line in graph). See Supplemental Table 8 for full jackknife results and Methods for discussion of further robustness testing.
