Inference of RhoGAP/GTPase regulation using single-cell morphological data from a combinatorial RNAi screen

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Figure 3.
Figure 3.

Point sets for RhoGTPase overexpression TCs and corresponding phase space. Point sets for RhoGTPase overexpression TCs and classification of all single cells in the double-knockdown screen. (A) Point sets for RhoF30L (blue), RacF28L (green), and Cdc42Y32A (red) shown in reduced-dimensional feature space. (B) The mapping of all 6480 single cells from the double-knockdown RhoGAP screen to RhoGTPase overexpression TCs. In effect, the classification model defines a phase space for mapping single cells in the set of upstream TCs to the set of downstream TCs.

This Article

  1. Published in Advance February 9, 2010, doi: 10.1101/gr.100248.109 Genome Res. 20: 372-380

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