Figure 9.
Effect of ATRX RNAi-knockdown on telomeric association of CBX5 in mouse ES cells. (A–C) ES129.1 cells transfected with scramble RNAi oligonucleotide (A) and ATRX-specific RNAi oligonucleotide sets (B–C; set 3 and sets 1–3 [Invitrogen]) for a period of 48 h. Transfection of ES129.1 cells with scrambled RNAi oligonucleotide did not affect localization of CBX5 (Aiii, red) at the telomeres (arrows), as indicated by costaining with TERF1 (Aii, green). However, RNAi depletion of ATRX with RNAi oligonucleotide sets, set 3 (B) and sets 1–3 (C) led to a significant dissociation of CBX5 from the telomeres (arrowheads) in ES129.1 cells.
