A new strategy for genome assembly using short sequence reads and reduced representation libraries

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Figure 8.
Figure 8.

Our fly was compared with the dm3 reference across various annotated regions using SSAHA-SNP. Within annotated regions the variation rate matches previous Drosophila variation findings. The most conserved regions were the first (Pos1) and second (Pos2) codon positions, conserved sequence (Cons), and splice-site junctions (SplSt). The most variable region was the third codon position (Pos3). For comparison, annotated protein-coding regions (Coding), 5′ untranslated regions (5UTR), 3′ untranslated regions (3UTR), noncoding sequence (NC), and conserved noncoding sequence (Cons NC) were included.

This Article

  1. Genome Res. 20: 249-256

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