Figure 5.
Cohesin loading occurs at ORC binding sites independent of pre-RC assembly. (A) ORC2 and SMC1 binding sites colocalize. ORC2 and SMC1 binding are indicated as blue density tracks for a 300-kb region of chromosome 2L. (B) ORC appears to nucleate cohesin binding. The average enrichments for ORC2 and SMC1 are plotted relative to the peak of all SMC1 binding sites in the Drosophila genome. (C) Cohesin loading is independent of pre-RC formation. Chromatin-associated MCM2-7, ORC, acetylated H4, and cohesin subunits at various points in the cell cycle (Supplemental Fig. 4).
