Figure 1.
ORC localizes to specific chromosomal locations. (A) A subset of ORC binding sites map to early origins of replication. (Black) MA2C-normalized log2 ratios of ORC2 enrichment from chromatin immunoprecipitations are plotted as a function of chromosomal position for a 600-kb window of chromosome 3R. (Gray) Early origins were identified by immunoprecipitation of BrdU-containing replication intermediates during an HU arrest. (B) ORC is enriched at early origins of replication. The summits of 630 HU-resistant early origins were determined, and the average BrdU (gray) or ORC (black) enrichment is plotted relative to the distance from the early origin summit.
