BRM promotes the interaction of JRA and GAF with their binding motifs through enhancement of chromatin accessibility. (A) Depletion of BRM causes a reduction in the correlation between binding and recognition motif occurrence for GAF and JRA, indicating that BRM enhances binding of these DBFs to their recognition motifs. cntrl, control knockdown; brm kd, brm knockdown. (B) Depletion of BRM causes a slight decrease in chromatin accessibility at BRM target genes. Depicted are changes in accessibility per DpnI-fragment that overlaps with indicated target or nontarget reporters of microarrays used for DamID profiles. (C) Same as B, but for GAF and JRA target genes that are either BRM targets or nontargets. (D) Cartoon model depicting the targeting of GAF through the combined action of BRM and GAGA motifs. A similar model may apply to JRA and SU(VAR)3-7. P-values are according to a test for difference between two dependent correlations (Chen and Popovich 2002) (A) or two-sided Wilcoxon tests (B,C).
