Figure 4.
BRM mediates targeting of GAF, JRA, and SU(VAR)3-7. (A) Western blot of BRM protein after RNAi knockdown of brm. RNAi of white (w), a gene that is not expressed in Kc cells, served as a control. Asterisk marks a nonspecific band. Lamin was probed as a loading control. (B–D) Changes in binding levels of GAF, JRA, and SU(VAR)3-7 at BRM target genes (black) and nontarget genes (gray) upon knockdown of brm, relative to control knockdown. (E) No significant changes in HP1 relocation after brm knockdown. (F) brm knockdown does not affect SU(VAR)3-7 binding at heterochromatic genes, defined as HP1 target genes. P-values are according to Wilcoxon tests.
