Evidence of post-transcriptional cleavage from full-length cDNA transcripts. (A) Schematic diagram illustrating the mapping of 5′-cDNA termini that span exon–exon junctions (EEJs) and 3′-cDNA termini to the annotated 3′ end of the host RefSeq gene. (B) Cumulative frequency distribution of mouse exonic CAGE tags within a 100-nt window cantered on the 5′ termini of exonic cDNAs. (C) Cumulative frequency distribution of 3′-cDNA termini distribution centered on a 100-nt window centered on the annotated 3′ end of the host RefSeq gene. (D) Cumulative frequency distribution of polyadenylated events within a 2-kb window centered on exonic CAGE tags. RNA sequencing library derived from mouse brain (Parkhomchuk et al. 2009).
