Expression analysis of AK005651 in congenic NOD mouse strains. Quantitative real-time PCR was performed to detect expression differences between mouse strains at 50 d of age for the spliced product derived from the following: (A) exons 4 and 5, which are present in both splice variants; (B) exons 2 and 3; (C) exons 2 and 4. Different cohorts of NOD, B6, and Idd11D mice to those in Figure 3 were generated and used in conjunction with Idd11B, Idd11E, Idd11F, and Idd11G mice for this experiment. Fold change (≥2) is shown only for significant pairwise comparisons between NOD and other mouse strains (P < 0.05, adjusted for multiple testing). Bars, mean expression level (±pooled SEM for each tissue).
