Schematic diagram and expression analysis of AK005651. (A) The Idd11 haplotype (variants 1–5) is located within a predicted gene, termed AK005651, consisting of five exons (Supplemental Table 5). Exon 3 is alternatively spliced giving rise to two different transcripts (±exon 3). Quantitative real-time PCR was performed to detect expression differences between mouse strains at 50 d of age for the spliced product derived from: (B) exons 4 and 5, which are present in both splice variants; (C) exons 2 and 3, (*) one of the four C57BL/6 samples was an extreme outlier (the mean relative normalized expression is 2.29 excluding this outlier); (D) exons 2 and 4. Approximate fold change (≥2) is shown only for significant pairwise comparisons between NOD and other mouse strains (P < 0.05, adjusted for multiple testing). Bars, mean expression level (±pooled SEM for each tissue).
