Three genomic enrichment methods. (A) (MIP) Molecular Inversion Probe: 70 base probes are prepared and hybridized to genomic DNA. Capture occurs by filling in sequence between the probe-targeting arms with polymerase and then sealing the circle with ligase. Total genomic DNA is removed with nucleases. The remaining closed circles undergo shotgun library and sequencing library preparation, followed by sequencing. (B) (SHS) Solution Hybrid Selection: A sequencing library is prepared from genomic DNA. This library is hybridized to biotinylated RNA probes in solution and recovered with streptavidin beads. Eluted products are amplified prior to cluster generation and sequencing. (C) (MGS) Microarray-based Genomic Selection: A sequencing library is prepared from genomic DNA and hybridized to a capture array. Eluted products are amplified prior to cluster generation and sequencing.
