Unambiguous adenine addition is reduced in miRNA bound to EIF2C2 and EIF2C3 proteins. Seventy-six miRNA loci contained significant amounts of 3′ unambiguous adenine addition in the control library. The percentage change of addition in these loci across the EIF2C1, EIF2C2, and EIF2C3 libraries are depicted as barplots with each bar representing one of the 76 loci. Loci are sorted by order of increasing percentage change; therefore, a bar in one plot does not necessarily represent the same miRNA locus as the corresponding bar in another plot. For the list of miRNA loci with names and percentage reductions, please see Supplemental Tables S12, S13. Bars corresponding to loci with significant reduction or increase in the AGO libraries are colored orange.
