PAPD4 broadly affects adenine addition at THP-1 miRNA loci. Comparison of unambiguous adenine addition rates (A) and ambiguous adenine addition rates (B) across control and PAPD4, PAPD5, and PAPD7 knockdown short RNA libraries. Lower left panels plot the percentage of adenine addition at specific loci between libraries, which are labeled on the diagonal panels. Loci with <50 sequence tags in the control condition are excluded to reduce noise. Upper right panels give the Pearson's correlation between two libraries. Loci with significant decrease (Fisher's exact test, Bonferroni post hoc correction) in addition relative to control library are colored in orange. (C) Percentage of reduction in adenine addition at miRNA loci in the PAPD4 knockdown library relative to control library for miRNA loci which meet the following two requirements: (1) reduction relative to control library is significant, and (2) addition is significantly greater than the expected error rate of addition (see Methods).
