Figure 5.
H2A.Z abundance in wild-type (WT), MYC-transformed, and tumor cells. (A) Western blots for H3 (above) and H2A.Z (below) for the soluble extracted (Input) material and the insoluble pellet. The antibody against histone H3 recognizes H3.1, H3.2, and H3.3 and therefore detects essentially all nucleosomes, regardless of the percentage of cells in S phase. (B) The ratio of total H2A.Z protein over H3, which includes both soluble and pellet fractions. (C) Expression of H2A.Z and MYC in the three cell types by microarray analysis (two replicates of each). (*) Expression changes that were significant at the P < 0.0001 level using Cyber-T.
