Stress-regulated alternative splicing of Arabidopsis splicing modulator ATSRP30. (A) Known isoforms (blue) and TAU-predicted variants (orange) of the ATSRP30 gene. (B) Predicted ATSRP30 protein domain structures (RRM, RNA recognition motif; SR, serine/arginine rich domain) and polypeptide sizes. (C) Quantification of accumulation of full-length ATSRP30 (reference isoform 1) and a PTC-containing isoform (4) by qRT-PCR under various abiotic stresses. Note the significant shift in the relative isoform ratio under high light, heat, and salt treatments. Relative expression levels were calculated using −ΔΔCt method (Livak and Schmittgen 2001) and EF-1-ALPHA mRNA as an internal reference control.
