Acetaminophen (APAP)-induced hepatotoxicity in inbred mouse strains. (A) Serum alanine aminotransferase (ALT) activity was measured 6 h after 16 inbred mouse strains were treated with 300 i.p. mg/kg APAP. The bars represent the average ± SD for N = 5 mice per group. SJL/J mice were resistant to APAP-induced toxicity, while all other strains developed significant toxicity. (B) Serum ALT (left) and the percentage of moribund mice (right) were measured at the indicated times after APAP treatment in four inbred strains. Veterinarians that were blinded to whether the mice received drug or placebo determined when mice were moribund. The ALT measurements are the average ± SE (N = 7 mice per group); and the percentage of moribund mice was determined from analyses of 10 mice. (C,D) Hematoxylin and eosin staining of liver tissue from SJL/J and C57BL/6J mice at 0 and 6 h after APAP treatment are shown at 100× and 400× magnifications, respectively.
