Figure 2.
Improvement of padlock capturing efficiency with longer hybridization time (left), more probes (middle), and appropriate dNTP concentration (right). The ratios (10:1, 50:1, 100:1, and 250:1) are molar ratios between each of the padlock probes and genomes. dNTP (1×) is defined as the minimum amount of dNTP needed to capture all genomic copies at each target region. The fold improvement (vertical axis at right) is relative to the reaction with 10:1 probe ratio, hybridized for 15 min at 60°C, and with 100× dNTP. Similar results were observed in independent experiments.
