Endonuclease-sensitive regions of human spermatozoal chromatin are highly enriched in promoter and CTCF binding sequences

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Figure 1.
Figure 1.

Analysis of DNA fractions obtained by SRD and MND treatment of sperm nuclei. All DNA samples were resolved on 1.8% agarose gels. (Lane 1) A 0.4–10-kb DNA ladder. (Lane 2) Total (unfractionated) DNA (NF). (Lane 3) The soluble DNA released after extraction of sperm nuclei with 0.65 M NaCl followed by digestion with BamHI and EcoRI (SRDS). (Lane 5) The corresponding insoluble pellet (SRDI). (Lane 4) DNA released from sperm after MNase digestion (MNDS). (Lane 6) The corresponding insoluble pellet (MNDI).

This Article

  1. Published in Advance July 7, 2009, doi: 10.1101/gr.094953.109 Genome Res. 19: 1338-1349

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