Efficient frequency-based de novo short-read clustering for error trimming in next-generation sequencing

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Figure 2.
Figure 2.

Distribution of base quality QVs given by Illumina of small RNA reads after trimming of 3′ linker sequences. (A) Average of given Illumina QVs at each base position. (B) Expected Illumina error rate P at each base. P is calculated from Q (the Illumina QV) at each position according to the formula: Perror = 1/[1 + 10(Q/10)].

This Article

  1. Published in Advance May 13, 2009, doi: 10.1101/gr.089151.108 Genome Res. 19: 1309-1315

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