Figure 1.
The problem of nonunique genotypes in simple row/column strategies. (A,B) Pooling and arraying of 20 specimens. Letters indicate pools of rows, and Roman numerals indicate pools of columns. (A) The (red) mutant state is unique and appears only in specimen #6 (left). After sequencing the pools (right), this genotype is found in pool II and B, a pattern that can only be associated with specimen #6, demonstrating successful decoding. (B) The mutant state appears in #6 and #20. After decoding there is an ambiguity of whether the mutant state is associated with specimens #6 and #20, or #8 and #18, or #6, #8, #18, and #20.
