Monitoring mRNA expression by qRT-PCR in linezolid-resistant Streptococcus pneumoniae. Two genomic regions are shown, one containing the ABC protein gene spr1021 (A) and one containing the ABC protein genes spr1887 and spr1885 (B). Between spr1887 and spr1885 there is a putative ORF, although it is not clear whether it is real. The spr# is according to the nomenclature of strain R6. The RNAs were normalized with 16S rRNA. The expression ratios shown are the cycle threshold (Ct) ratios between the resistant cells and the parent wild-type cells for each individual gene shown. Possible sites of transcription initiation (arrows) and termination (vertical bars) of the operons are indicated. Values represent an average of three independent experiments.
