Human miRNA hsa-miR-605 and SEC24D. (A) Predicted sites on the 5′-UTR and 3′-UTR targeted by the 3′-end and 5′-end, respectively, of hsa-miR-605. (B) Western blot analysis of SEC24D. Protein extract (40 μg) from three days post-transfected HeLa cells was separated by SDS-PAGE and probed with anti-SEC24D monoclonal antibody, with actin (ACTB) as control. (C) Densitometric analysis of the Western blots. X-ray films were scanned with HP Scanjet 3570c (Hewlett-Packard) and quantified using NIH image software. (D) SEC24D mRNA expression fold change in HeLa cells two days post-transfection with Negative Control-1, Pre-mir-605, or anti-miR-605. Error bars represent standard deviation from a triplicate experiment.
