Human miRNA hsa-miR-34a and target WNT1. (A) Predicted interactions between hsa-miR-34a and WNT1 UTR sequences. Extended seed match between the 5′-end of miR-34a and the 3′-UTR binding site is shown in bold red. Interactions between the 3′-end of miR-34a and the 5′-UTR inserted sequences are shown in bold blue. (B) Schematic of vector constructs containing firefly luciferase reporter gene used in transfection experiments. 5′-UTR and 3′-UTR inserts are indicated as 5U and 3U, respectively. (C) Effect of 5′-UTR site in wild-type and mutant form on luciferase expression when treated with miR-34a. Renilla-normalized luciferase expression was normalized with negative control RNA oligo. Error bars represent standard deviation from triplicate experiments.
