CpG methylation-dependent enhancer activity of selected DMRs. Several DMRs were cloned upstream of a basic EF1-promoter into the CpG-free luciferase vector pCpGL-P. The indicated plasmids were in vitro SssI-methylated (mCpG) or unmethylated (CpG) and transiently transfected into Jurkat T cells that were left untreated (A), or were stimulated with PMA and ionomycin (B) or PHA (C) after transfection. Luciferase activity was normalized against the activity of a cotransfected Renilla construct, and mean values ± SD are shown relative to the unmethylated pCpGL-P. (*) Significant difference between methylated and unmethylated plasmids (P < 0.05, paired Student's t-test).
