Structural context of the H2A.Z nucleosome over-digestion. (A) Nucleosome core particle structure containing H2A.Z variant (PDB_ID 1f66) (Suto et al. 2000) with only half of nucleosomal DNA (cyan) and one set of the histone proteins (white and magenta) shown for clarity. The major H2A histone (dark blue) from the best resolved nucleosome structure (PDB_ID 1kx5) (Davey et al. 2002) is superimposed onto the H2A.Z variant (magenta) for comparison. Base pair at position −43 (yellow) marks the 30-bp shortening of the protected DNA fragment. (B) Detailed view of the H2A.Z structure fragment marked with the box in A. Note that the N-terminal tail of the H2A.Z histone was not included in the published structure as too disordered (Suto et al. 2000).
