swi*603 strain pedigree and single base changes detected. (A) swi*603 strain DI36 was backcrossed twice. The DI36 strain, our wt (DG21/FY648), and strains derived from the two crosses were sequenced to various degrees of coverage. (B) Single base changes identified by 200-fold resequencing of 972h− by the Broad Institute, 36-fold sequencing of DG21 (wild type), and 43-fold sequencing of swi*603 strain DI36 were compared to identify 73 DI36-specific high-confidence single base changes potentially responsible for the observed phenotypes. In addition, divergences between wt DG21 and the recently sequenced 972h− were identified relative to the cosmid library-derived wt reference sequence (Wood et al. 2002).
