Selecting genes for the insulin resistance siRNA screen. (A) Global view of how 313 genes were selected for screening. Genes from multiple sources were considered and filtered based on whether their protein products could be targeted by small molecules. (B) Distribution of sources from which the 313 genes were selected. For example, 177 of 313 genes were selected because they were supported as causal for diabetes/obesity in an experimental cross population. (C) Distribution of the 313 genes with respect to five function categories: (1) G protein–coupled receptor (GPCR), (2) protease, (3) ion channel, (4) kinase/protease, and (5) other function. The numbers of genes in each category are provided after category names, where (+) stands for a positive siRNA hit and (−) denotes a negative siRNA hit.
