Binding profiles, expression, and growth phenotypes link SBTFs to Hda1p. (A) SBTFs that have a significant percentage of targets in HAST domains (P < 0.01). Exact numbers of targets are above each bar. (B–D) Box-and-whisker plots of gene expression changes in an hda1Δ strain compared with wild type (data from Bernstein et al. 2002) for targets bound by Yap6p (P = 10−8) (B); Phd1p (P = 0.00006) (C); Yap1p (statistically unaffected) (D). (E) YAP6 and PHD1 display a condition-specific genetic interaction during growth on non-glucose carbon sources. Fivefold serial dilutions were grown on synthetic complete (SC) medium supplemented with 2% of one of the following carbon sources: glucose, galactose, ethanol, or glycerol. Controls are BY4741, the parent strain used to create gene deletions, and gal4Δ, which does not grow on galactose.
