Dynamic reprogramming of transcription factors to and from the subtelomere
- 1 Division of Biological Sciences, University of California San Diego, La Jolla, California 92093, USA;
- 2 UCSD Moores Cancer Center, University of California San Diego, La Jolla, California 92093, USA;
- 3 Department of Bioengineering, University of California San Diego, La Jolla, California 92093, USA
Abstract
Transcription factors are most commonly thought of as proteins that regulate expression of specific genes, independently of the order of those genes along the chromosome. By screening genome-wide chromatin immunoprecipitation (ChIP) profiles in yeast, we find that more than 10% of DNA-binding transcription factors concentrate at the subtelomeric regions near to chromosome ends. None of the proteins identified were previously implicated in regulation at telomeres, yet genomic and proteomic studies reveal that a subset of factors show many interactions with established telomere binding complexes. For many factors, the subtelomeric binding pattern is dynamic and undergoes flux toward or away from the telomere as physiological conditions shift. We find that subtelomeric binding is dependent on environmental conditions and correlates with the induction of gene expression in response to stress. Taken together, these results underscore the importance of genome structure in understanding the regulatory dynamics of transcriptional networks.
Footnotes
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↵4 Corresponding author.
E-mail trey{at}bioeng.ucsd.edu; fax (858) 534-5722.
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[Supplemental material is available online at www.genome.org.]
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Article published online ahead of print. Article and publication date are at http://www.genome.org/cgi/doi/10.1101/gr.084178.108.
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- Received August 6, 2008.
- Accepted February 9, 2009.
- Copyright © 2009 by Cold Spring Harbor Laboratory Press
