Table 1.
Verification of ORFs identified by six-frame translation searches in three species
Click on table to view larger version.
-
aVerification was occasionally not attempted if the peptide used to identify the ORF was short, repetitive, flanked closely by in-frame stop codons with no apparent intervening splicing sites, or mapped back to many regions of the genome. Verification was judged unsuccessful if two rounds of RACE primer design (in each direction) failed to produce a complete transcript.
