Chromosome 6 homologs from metaphases belonging to the father of the proposita, (A) after Q-banding top and C-banding bottom; (B) hybridized with BAC clones RP11-28P11 and RP11-91K21, which flank the normal centromere; (C) hybridized with BAC clones RP11-58F2 and RP11-59N15, which flank the repositioned neocentromere. Note that marker order is conserved in the variant chromosome, thus excluding an inversion. The top part of B and C shows the DAPI banding of the homologs to better show the morphology of the chromosomes, the primary constriction in particular, indicated by the arrows. The map position of the BAC clones used in B and C is reported in Table 1. The white arrow indicates the repositioned centromere, while the green arrow indicates the normal centromere.
