Relative coverage of WormBase genes by position and strand. (A) Results of analyzing 7571 full-length transcripts, normalized for length and depth of coverage, show almost uniform coverage along the body of the gene, with a pronounced overrepresentation at the 5′ end and decreasing depth of coverage at the 3′ end. (YA) Young adult. (B) Average depth of coverage at the 5′ end by strand, measured in bases from the start of the transcript. This plot confirms the overrepresentation at the 5′ end and demonstrates that it is almost entirely restricted to the coding strand. (C) Average depth of coverage at the 3′ end, by strand, measured from the transcript end. Coverage on both strands falls approaching the 3′ end, with loss of the sense (s) strand coverage followed by decrease of the antisense (as) to less than half the average depth of coverage. Only data for L2 and L3 are shown for clarity.
