Deleting telomere anchoring pathways induces subtelomeric gene activation and misregulation of some internal genes. (A) Genes up-regulated in a yku70 esc1 (GA-2768) strain overlapped significantly with subtelomeric genes (defined as located <20 kb away from a telomere, P = 10−5). Genes down-regulated in yku70 esc1 did not overlap significantly with subtelomeric genes (P = 0.1). (B) The chromosomal position of all genes whose expression increased (red) or decreased (green) >1.5-fold between wild-type and yku70 esc1 strains is shown. Boxes indicate clusters of regulated genes. (C) Significance of expression changes as a function of distance to chromosome end. For each gene, a significance (Z-value) for its expression change in the yku70 esc1 mutant was calculated and the figure shows average Z-values as a function from distance to the nearest chromosome end. Positive values indicate up-regulation and negative values down-regulation.
