Figure 2.
GBD fusions can promote silencing independently of SIR distribution by having a sufficiently strong affinity for the SIR complex. (A) Targeted silencing is monitored for the hmr∷e-URA3-I reporter as in Figure 1 in GA-1998 (WT) and its yku70 deletion derivative (GA-1997) expressing different GBD fusions as indicated. (B) Bar graph representing the percent of cells shown in A growing on 5-FOA, for four independent transformants for each GBD fusion. (C) Immunolocalization of Sir4 and nuclear pores (MAb414) in the strain GA-1997 (yku70), expressing GBD–Yku70 or GBD–Yku80, as indicated. Bar = 2 μm.
