Figure 7.
Analysis of CHD7 sites in reporter assays. (Top) Genomic fragments (red) were cloned downstream from the luciferase gene (blue) driven by the minimal Sox9 promoter (orange). (Bottom) Compared to cells transfected with constructs containing genomic inserts devoid of CHD7 binding in DLD1 cells (non-CHD7 sites, orange bars), 3/6 constructs containing CHD7 sites (blue bars) show significant increases in luciferase activity when transfected into DLD1 cells. The asterisks denote significance as determined by two-tailed t-tests. The red horizontal line denotes the average of the non-CHD7 sites.
