Detecting heterozygosity in shotgun genome assemblies: Lessons from obligately outcrossing nematodes

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Figure 5.
Figure 5.

Changes in allele frequencies in (A) fog-1 and (B) sur-2 loci in C. brenneri. Gray represents allele “A” and black represents allele “B.” Frequencies in LKC28 and “PB2801 present” were obtained by PCR genotyping of individual animals from these strains—44 and 76 for fog-1 and 57 and 50 for sur-2. Allele frequencies in the strain PB2801 at the time of sequencing were inferred from the average number of sequence reads through each of the two alleles.

This Article

  1. Genome Res. 19: 470-480

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