Figure 7.
N. crassa performs efficient de novo methylation of heterochromatic regions. The distribution of 5mC is shown across LGVII for wild type (wt), the methylation deficient strains dim-2 and hpo, and wild-type daughter strains from two crosses between methylation deficient strains. Enrichment values for MeDIP experiments are shown as log2 values on the y-axis. The wild-type strain N3668 was obtained by crossing two strains that lack 5mC but retain H3K9me3, dim-2, and hpo. The wild-type strain N3669 was obtained by crossing two strains that lack both H3K9me3 and 5mC, dim-5, and dim-8. Thus, all methylation in these strains is a result of de novo methylation.
