Distribution of H3K9 methylation in wild-type, hpo, and dim-2 strains. (A) The distribution of H3K9me3 across LGVII is shown for wild-type, dim-5, hpo, and dim-2 strains. (B) The distribution of H3K9me3 is plotted for wild-type, hpo, and dim-2 strains for LGVII 5mC peak 40, (C) the previously identified methylated region 8:B1, and (D) LGVII 5mC peak 33 (Supplemental Table 1 and Selker et al. 2003). Conventional ChIP was performed using antibodies to H3K9me3 for the indicated strains. For each strain, PCR products obtained using the immunoprecipitated fraction (H3K9me3, top) and whole cell extract (WCE, bottom) as a template were resolved by gel electrophoresis. The black bars numbered P2–P7 depict the position of the expected PCR products within each region. The coding region of histone H4 (hH4) was used as an internal euchromatic control. The labels to the right of each autoradiogram indicate specific PCR products. The relative enrichment of each PCR product is shown below.
