Tajima's relative rate tests on paired LWS sequences using a χ2 distribution and one degree of freedom, with the fruit fly Rh4 sequence as an outgroup
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(ZFL) Zebrafish LWS; (GFL) goldfish LWS; (ESL1) elephant shark LWS1; (ESL2) elephant shark LWS2; (PLL) pouched-lamprey LWS. Statistical significance showing that the null-hypothesis, that the relative rate of nucleotide substitution along one lineage equals that of the sister lineage, should be rejected is indicated by (*) P < 0.001, (**) P < 0.01, and (***) P < 0.05.











