
Absorption spectra of regenerated C. milii LWS1 (left) and LWS2 (right) mutants. (Top) His181Tyr single substitutions in both LWS1 and LWS2 pigments. (Middle) Ser292Ala (LWS1) or Ala292Ser (LWS2) single substitutions. (Bottom) His181Tyr and Ser292Ala (LWS1) or His181Tyr and Ala292Ser (LWS2) double substitutions. For all pigments, representative difference spectra are shown that were fitted to Govardovskii et al. (2000) templates to determine the λmax values. The vertical dotted line shows the relative position of the spectral peak values for both wild-type LWS1 (499 nm) and LWS2 (548 nm) regenerated pigments.











