Cell-specific activity of conserved FOXA1 recruitment sites. (A) Comparison of FOXA1 binding sites identified in MCF7 and LNCaP cells by ChIP-chip (Lupien et al. 2008). (B) FOXA1 binding sites shared between MCF7 and LNCaP and harboring low FAIRE enrichment in MCF7 cells were selected. These sites were then classified relative to their FAIRE enrichment levels (low, medium, or high) in LNCaP cells. (C) Histogram indicating the percentage of sites defined in (B) that also recruits AR in LNCaP cells. ** Indicates a statistically significant difference (P < 0.01) between FOXA1 binding sites with high and low FAIRE enrichment in LNCaP cells. (D) FOXA1 binding sites shared between MCF7 and LNCaP and harboring low FAIRE enrichment in LNCaP cells were selected. These sites were then classified relative to their FAIRE enrichment levels (low, medium, or high) in MCF7 cells. (E) Histogram indicating the percentage of sites defined in (B) that also recruits ESR1 in MCF7 cells. *** Indicates a statistically significant difference (P < 0.001) between FOXA1 binding sites with high and low FAIRE enrichment in MCF7 cells.
