Figure 1.
Men ε/β ncRNA localization in C2C12 nuclei. (A) RNA FISH revealed that the Men ε/β transcripts are localized in discrete foci in C2C12 myoblast nuclei. (B) In C2C12 myotubes, the foci of Men ε/β ncRNAs are enlarged and present in greater numbers than in C2C12 myoblasts. Scale bars, 10 μm. (C) Both Men ε and Men β isoforms are up-regulated upon C2C12 myoblast differentiation into myotubes as assessed by Q-PCR. Gapdh was used as a normalization control. The data in the histogram are shown as mean and standard deviation values of three independent experiments.
