Large-scale RACE approach for proactive experimental definition of C. elegans ORFeome

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Table 3.

  • Positive cloning of RACE products were deduced from the presence of recombined Gateway tags, rather than the presence of high phred score insert sequences since multiple splice forms in minipools could generate mixed unreadable sequence reads. Sequences eliminated by minipool filters were either unreadable in the supposed insert region, or had gaps between the Gateway tag and insert sequence.

This Article

  1. Published in Advance October 2, 2009, doi: 10.1101/gr.098640.109 Genome Res. 19: 2334-2342

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