
PCR rescue success versus target size. (Black bars) The number of assigned targets in each size range; (white bars) the number of assigned targets that were obtained as full-CDS clones, with the number of clones recovered shown above the bars. The triangles and trendline show the percentage recovered for each size group. Excluded from these calculations are RefSeq targets where the assigned CDS later was changed, suppressed, or withdrawn over the course of the PCR rescue program. Among 8764 human and mouse targets with changed annotation, we obtained a full-CDS clone for 3197 (36%), including one 10.8-kb clone (BC150731).











