Influence of thiamin (A) and sugar concentration (B,C) on the specific growth rate (see Methods) of the diploid S288C strain GSY157 (black circles) or the industrial strain CAT-1 (red triangles) in synthetic defined media lacking pyridoxine and thiamin (B), or the same media supplemented with thiamin (A,C). (D) Competition between the diploid S288C strain GSY157 (black circles) and the CAT-1-derived industrial strain EHD04 (red triangles) during sequential growth (see Methods) in synthetic defined media lacking pyridoxine and supplemented with thiamin. Unless otherwise indicated, the concentrations of glucose and thiamin in the medium were 200 g/L and 3 μM (1 mg/L), respectively.
