Industrial fuel ethanol yeasts contain adaptive copy number changes in genes involved in vitamin B1 and B6 biosynthesis

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 3.
Figure 3.

Typical growth curves of the diploid S288C-derived laboratory yeast strain GSY157 (A), the industrial fuel ethanol CAT-1 strain (B), as well as the diploid laboratory ura3/ura3 yeast strain FY1679 transformed with a multi-copy 2μ URA3+ control (no-insert) plasmid (C), or with the same plasmid containing a cloned SNO/SNZ gene pair from strain S288C (pBD1001) (D), in synthetic defined media (see Methods) containing 200 g/L glucose and lacking both pyridoxine and thiamin (green), or the same media supplemented with 1 mg/L of thiamin (blue).

This Article

  1. Genome Res. 19: 2271-2278

Preprint Server