Figure 2.
Detection of SNO2/3 genes in the industrial fuel ethanol yeasts. (A) Separation of chromosomes of yeast strains by PFGE; gel stained with ethidium bromide. (B) Southern blot of gel shown in panel A, hybridized with a probe for SNO2/3 to detect which chromosomes carry SNO2 or SNO3 genes. (Lane 1) Reference strain S288C, which contains SNO2 on chromosome XIV and SNO3 on chromosome VI (also indicated to the right of panel B); (lanes 2–6) strains CAT-1, PE-2, VR-1, BG-1, and SA-1, respectively. Roman numerals to the left of panel A are the chromosome numbers corresponding to the S288C chromosome bands (note that many are seen as doublets).
