Band-array analysis of Chr6L and Chr6S homologs in JAY270. The curves indicate the normalized hybridization signal of specific chromosomal DNA samples (y-axis) to probes arranged according to their chromosomal coordinates (x-axis) in S288c (A) Chr6 and (B) Chr1. The dashed vertical lines indicate the breakpoints where JAY270 Chr6S and Chr6L differ from each other and from the S288c chromosomes. Only the data for Chr6 and Chr1 probes are shown. No hybridization signal was detected for other S288c chromosomes, with the exception of the right end of Chr8, which is essentially identical to Chr1. SEC53 and RPN12 indicate the most distal essential genes in S288c Chr6, and SNO3, SNZ3, AGP3, and YAR064W indicate the position of Southern blot probes used in Figure 4. The signal valley in the central position of Chr6 corresponds to the position of the YFLWTy2-1 retrotransposable element (arrow) that is not present in the JAY270 Chr6 homologs. (Black circles) Centromere positions.
