Molecular karyotype of meiotic products and segregation of chromosomal rearrangements. (A) Ethidium bromide staining of a PFGE including the four meiotic spore clones from a JAY270 tetrad. The S288c-isogenic control strain used was the JAY309 diploid. Southern hybridizations of the PFGE in A using as probes the (B) AGP3, (C) YAR064W, and (D) SNO/SNZ sequences that were PCR-amplified from S288c genomic DNA. The SNO/SNZ (2-3) probe detects only the duplicated SNO2, SNZ2, SNO3, and SNZ3 genes. The diverged single-copy genes SNO1 and SNZ1 on Chr13 are not detected. The numbers to the left indicate the S288c chromosomes to which these probes hybridized. The YAR064W gene is duplicated in S288c and JAY270 at the right end of Chr8.
